%0 Journal Article %T Cystathionine gamma lyase aggravates orthodontic root resorption in mice %A Lu, Caizhu %A Chen, Liyuan %A Hua, Yongmei %J Annals of Translational Medicine %D 2019 %B 2019 %9 %! Cystathionine gamma lyase aggravates orthodontic root resorption in mice %K %X Background: This study aimed to determine the contribution of cystathionine gamma lyase (CSE) to physiological and orthodontic root resorption in mice. Methods: Mice genetically deficient in the CSE (CSE -/- ), the dominant hydrogen sulfide (H2S)—generating enzyme in osteoclast were used in this study. Physiological and orthodontic root resorption was assessed with micro computed tomography (micro-CT) and scanning electron microscopy (SEM) in the mice at the age of 8-, 26-, and 52-week and in 8-week old mice following 1-, 2-, and 3-week orthodontic treatment, respectively. Hematoxylin and eosin (HE) and tartrate-resistant acid phosphatase (TRAP) staining were used for further determination of root resorption and the number of osteoclasts. The receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin (OPG) level in surrounding alveolar bone of the maxillary first molar after 2-week orthodontic treatment was measured by reverse transcription polymerase chain reaction (RT-PCR). Results: Root resorption lacunae (RRL) gradually and significantly increased with age in wild type (WT) and CSE -/- mice. The CSE -/- showed less RRL compared with the WT group. At each time point of orthodontic treatment, the CSE -/- group had less RRL and osteoclasts than the WT group. The orthodontically induced RANKL/OPG mRNA expression in the periodontal tissue in the CSE -/- group was lower than that in the WT group. Conclusions: CSE contributes significantly to physiological and orthodontic root resorption. %U https://atm.amegroups.org/article/view/33051 %V 7 %N 23 %P 787 %@ 2305-5847