@article{ATM33051,
author = {Caizhu Lu and Liyuan Chen and Yongmei Hua},
title = {Cystathionine gamma lyase aggravates orthodontic root resorption in mice},
journal = {Annals of Translational Medicine},
volume = {7},
number = {23},
year = {2019},
keywords = {},
abstract = {Background: This study aimed to determine the contribution of cystathionine gamma lyase (CSE) to physiological and orthodontic root resorption in mice.
Methods: Mice genetically deficient in the CSE (CSE-/-), the dominant hydrogen sulfide (H2S)—generating enzyme in osteoclast were used in this study. Physiological and orthodontic root resorption was assessed with micro computed tomography (micro-CT) and scanning electron microscopy (SEM) in the mice at the age of 8-, 26-, and 52-week and in 8-week old mice following 1-, 2-, and 3-week orthodontic treatment, respectively. Hematoxylin and eosin (HE) and tartrate-resistant acid phosphatase (TRAP) staining were used for further determination of root resorption and the number of osteoclasts. The receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin (OPG) level in surrounding alveolar bone of the maxillary first molar after 2-week orthodontic treatment was measured by reverse transcription polymerase chain reaction (RT-PCR).
Results: Root resorption lacunae (RRL) gradually and significantly increased with age in wild type (WT) and CSE-/- mice. The CSE-/- showed less RRL compared with the WT group. At each time point of orthodontic treatment, the CSE-/- group had less RRL and osteoclasts than the WT group. The orthodontically induced RANKL/OPG mRNA expression in the periodontal tissue in the CSE-/- group was lower than that in the WT group.
Conclusions: CSE contributes significantly to physiological and orthodontic root resorption.},
issn = {2305-5847}, url = {https://atm.amegroups.org/article/view/33051}
}