%0 Journal Article %T Hes1 is associated with long non-coding RNAs in colorectal cancer %A Zhang, Yuqin %A Zheng, Lin %A Lao, Xuejun %A Wen, Mingbo %A Qian, Zhipeng %A Liu, Xin %A Tang, Hui %A Gao, Fei %J Annals of Translational Medicine %D 2019 %B 2019 %9 %! Hes1 is associated with long non-coding RNAs in colorectal cancer %K %X Background: Long noncoding RNAs (lncRNAs) play important roles in the development and pathophysiology of colorectal cancer (CRC). Our previous study showed that Hes1 was involved in the self-renewal and tumorigenicity of stem-like cancer cells in CRC. Methods: ArrayStar Human LncRNA/mRNA Expression Microarray Version 3.0 was used to detect lncRNA expression in CRC tissues compared with their matched non-tumoral tissues. RNA-binding protein immunoprecipitation and sequencing (RIP-seq) assay was used to detect lncRNAs binding to Hes1. Real-time qPCR was used to detect expression of specific lncRNAs in CRC tissues. Results: We found significantly up-regulated as well as down-regulated lncRNAs in CRC tissues compared with their matched non-tumoral tissues. We also screened a number of lncRNAs interacting with Hes1 in CRC cells. Interestingly, we found several lncRNAs binding to Hes1 (such as, GNAS-AS1, RP11-89K10.1, and RP11-465L10.10) were up-regulated in CRC tissues showed by the tissue microarray. Next, we confirmed that Hes1 directly interacted with these lncRNAs using RIP-qPCR and RNA pulldown assay. Finally, we verified the expression of these lncRNAs in 32 CRC samples as well as the adjacent non-tumoral tissues using real-time qPCR. Conclusions: Based on these, we speculate that Hes1 interacts with one or more lncRNAs which contribute to the development and progression of CRC. %U https://atm.amegroups.org/article/view/28737 %V 7 %N 18 %P 459 %@ 2305-5847