@article{ATM26600,
author = {Xiaotian Lei and Qinan Wu and Weiling Leng and Minxia Wu and Liu Chen and Ziwen Liang},
title = {Exenatide reduces cardiomyocyte apoptosis by stimulating adiponectin secretion and activating APPL1-AMPK-PPARα axis},
journal = {Annals of Translational Medicine},
volume = {7},
number = {14},
year = {2019},
keywords = {},
abstract = {Background: To explore the mechanism that exenatide reduces cardiomyocyte apoptosis via the adiponectin pathway in vitro.
Methods: Cardiomyocytes were randomly divided into the control group (group C), diabetic group (group D), diabetic + exenatide treatment group (group DE), diabetic + exenatide treatment + APPL1 overexpression group (group OE), and diabetic + exenatide treatment + APPL1 knock-down group (group KD). After 48 h culture, the apoptosis rate, the adiponectin level in the cell culture fluid, and the expression levels of APPL1, p-AMPK, PPARα and NF-κB were detected by TUNEL, ELISA, and Western blotting, respectively.
Results: Compared to group C, the apoptosis rate was markedly increased, the adiponectin level was decreased, the expression of APPL1, p-AMPK and PPARα was down-regulated and that of NF-κB was up-regulated in group D (P},
issn = {2305-5847}, url = {https://atm.amegroups.org/article/view/26600}
}