TY - JOUR AU - Han, Shuai-Shuai AU - Jiao, Qian AU - Bi, Ming-Xia AU - Du, Xi-Xun AU - Jiang, Hong PY - 2018 TI - The expression of K ATP channel subunits in alpha-synucleintransfected MES23.5 cells JF - Annals of Translational Medicine; Vol 6, No 10 (May 26, 2018): Annals of Translational Medicine (Focus on “Translational Neurodegeneration”) Y2 - 2018 KW - N2 - Background: SUR1, one of the subunits of ATP-sensitive potassium (K ATP ) channels, was found to be highly expressed in mRNA levels in the substantia nigra (SN) of Parkinson’s disease (PD) brains. Though the mechanism of the selective dopamine (DA) neurons death is still unknown, some studies have demonstrated that selective activation of the K ATP channels in the SN might be associated with the degeneration of DA neurons. The objective of our study is to examine the expressions of K ATP channel subunits in dopaminergic cells with alpha-synuclein (α-Syn) transfection. Methods: In this study, we detected the K ATP channel subunits mRNA levels in MES23.5 cells by real-time quantitative PCR after the cells transfected with α-Syn. Results: Our results showed that the mRNA levels of SUR1 subunit were markedly increased by 35% in WT α-Syn overexpression cells and by 31% in A53T α-Syn overexpression cells, respectively. However, the mRNA levels of SUR2B and Kir6.2 subunit have no obviously differences from the controls. Conclusions: We showed that the mRNA levels of SUR1 but not SUR2B or Kir6.2 were selectively upregulated in MES23.5 cells over-expressed with α-Syn. The findings demonstrated that the SUR1 overexpressed might be involved in the process of PD. UR - https://atm.amegroups.org/article/view/19200