AB008. SEPARATION and identification glycoprotein in human Fragile X syndrome serum
Part 2: Symposium

AB008. SEPARATION and identification glycoprotein in human Fragile X syndrome serum

Anh Luong1, Hoan Thi Phan1, Chi Van Phan2

1 Hanoi Medical University, Hanoi, Vietnam; 2Vietnam Academy of Science and Technology, Hanoi, Vietnam

Background: Fragile X syndrome (FXS), the most common cause of inherited mental retardation. The absence of FMRP results in FXS leading changes other proteins.

Objective: To detect changes of glycoproteins in human FXS serum.

Methods: Affinity chromatography with lectin concanavalin A (ConA) used to receive glycoproteins. These collected glycoproteins were then separated using 2-D electrophoresis. The protein spots were further excised, trypsin-digested, and analyzed by nanoLC couple with ESI MS/MS and identified by MASCOT v1.8 software.

Results and conclusions: Five glycoproteins showed the different expression levels in the FXS serum. Haptoglobin and IgJ were increased, Ceruloplasmin, Transferrin, Ig kappa were decreased. We found much useful information that related to FXS when these changed glycoproteins were analysed in detail.

Keywords: Fragile X syndrome (FXS); FMRP; FMR1; proteomics

Cite this abstract as: Luong A, Phan HT, Phan CV. SEPARATION and identification glycoprotein in human Fragile X syndrome serum. Ann Transl Med 2015;3(S2):AB008. doi: 10.3978/j.issn.2305-5839.2015.AB008

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