Perioperative crizotinib in a patient with stage IIIB ALK-positive non-small cell lung cancer: a case report
Case Report

Perioperative crizotinib in a patient with stage IIIB ALK-positive non-small cell lung cancer: a case report

Yu Tian1#, Jia Huang1#, Chongwu Li1#, Long Jiang1, Hao Lin1, Peiji Lu1, Qingquan Luo1, Guocai Yang2

1Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China; 2Zhoushan Hospital, Wenzhou Medical University, Zhoushan, China

#These authors contributed equally to this work.

Correspondence to: Guocai Yang. Zhoushan Hospital, Wenzhou Medical University, No. 739 Dingshen Road Lincheng New District, Zhoushan, China. Email:

Abstract: We present a case of a 43-year-old female patient with clinical stage IIIB (T3N2M0) anaplastic lymphoma kinase (ALK)-positive adenocarcinoma of the lung. Surgery was not performed initially because of multiple mediastinal lymph nodes invasion, although the mass was technically resected. With the assessment of upfront multidisciplinary consultation, administration of neoadjuvant crizotinib was selected to induce the downstaging and facilitate the subsequent surgical treatment. After 10 weeks of neoadjuvant crizotinib treatment, a partial response was achieved and the tumor could be radically resected. There were no sever toxic effects and treatment-related surgical delay during the whole neoadjuvant crizotinib therapy. The patient then successfully underwent video-assisted single port thoracoscopic right upper lobectomy and lymphadenectomy. Concurrent chemotherapy and radiotherapy were applied postoperatively. Perioperative targeted therapy demonstrated good curative effect in this case, and no recurrence was observed at the clinic 8 months after surgery. In this case, the safety and effectiveness of neoadjuvant crizotinib and subsequent surgery are preliminarily proved. We here intend to investigate the optimal setting of neoadjuvant targeted therapy combined with minimally invasive surgery and postoperative adjuvant therapy, inspire more potential targeted treatment based schedules and to apply these strategies in treating patients with locally advanced mutant-positive non-small cell lung cancer (NSCLC).

Keywords: Non-small cell lung cancer (NSCLC); stage IIIB; anaplastic lymphoma kinase-positive (ALK-positive); neoadjuvant therapy; case report

Submitted Apr 17, 2020. Accepted for publication Jun 11, 2020.

doi: 10.21037/atm-20-3927


Locally advanced non-small cell lung cancer (NSCLC) has always been recognized as a highly heterogeneous clinical issue (1). Concurrent chemoradiotherapy and surgery along with chemotherapy or chemoradiotherapy, as the two main treatment modalities, have been offered for patients with locally advanced disease. Although neoadjuvant chemoradiotherapy has been recommended as one of the clinical practices based on current National Comprehensive Cancer Network guidelines, a multimodality regimen for this malignant disease is still heterogeneous, and the prognosis remains unsatisfactory (2). In previous research, neoadjuvant therapy prior to surgical resection has been demonstrated to provide favorable tumor shrinkage and disease-free survival (DFS) for patients with locally advanced NSCLC (3-5).

The anaplastic lymphoma kinase (ALK) gene rearrangement is one of the significant driver mutations that can be detected in 4% to 6% of NSCLC patients (6). Enormous progress in efficacy and long-term survival has been achieved by applying ALK receptor tyrosine kinase inhibitors (TKIs) in advanced NSCLC (7). Crizotinib, as a typical ALK-TKI drug, was proven to be superior to combined chemotherapy in the first-line setting for advanced ALK-positive NSCLC by the PROFILE 1014 trial (8), with an improved objective response rate (ORR) (74% vs. 45%) and progression-free survival (PFS) (10.9 vs. 7 months). Furthermore, crizotinib has shown a more rapid 6-week response compared to the 12-week response of chemotherapy (9). However, research focusing on applying ALK-targeted treatment in locally advanced NSCLC or neoadjuvant therapy has rarely been reported, and the scant trials and studies that are available are limited in offering reliable evidence due their small sample sizes.

Thus, definite conclusions concerning the efficacy and optimal settings of neoadjuvant ALK-TKIs urgently need to be found. Here, we present a patient with stage IIIB ALK-positive NSCLC who obtained significant response to perioperative crizotinib treatment. Clinicopathological features of the patient are described in this article in accordance with the CARE reporting checklist (available at

Case presentation

A 43-year-old female patient was admitted with dry cough and a suspicious right upper lobe mass complicated by obstructive pneumonia. The patient represented maternal history of breast cancer. Personal history was negative for smoking and neoplastic diseases. Contrast-enhanced computed tomography (CT) scan (November 27, 2018) showed a 65.0 mm × 61.5 mm mass and obstructive symptoms, together with multiple enlarged hilar and mediastinal lymph nodes (Figure 1). The significant serum tumor markers included carcinoembryonic antigen (157.25 ng/mL), cancer antigen-125 (81.3 U/mL), and circulating cytokeratin 19 fragments (CYFRA21-1; 19.03 ng/mL). Positron emission tomography-CT (PET-CT) was performed revealing a high level of 18-fluorodeoxyglucose (FDG) uptake in the right upper lobe mass and right upper paratracheal nodes (2R) (Figure 2), which was highly indicative of malignant disease and lymph node (2R) metastasis with no signs of distant metastasis. A CT-guided percutaneous biopsy in the right lung neoplasm was performed. Pathological analysis showed a poorly differentiated adenocarcinoma. Immunohistochemistry revealed positive staining of cytokeratin (CK), CD56, and thyroid transcription factor-1 (TTF-1), and napsin A was partially positive. Gene mutation test was positive for ALK gene rearrangement. The patient was diagnosed with clinical stage IIIB (T3N2M0) pulmonary adenocarcinoma with hilar and mediastinal lymph node metastasis.

Figure 1 Prior to neoadjuvant immunotherapy, computed tomography (CT) images of the chest in November 27, 2018 indicated a 65.0 mm × 61.5 mm mass (★ marked) in the right upper pulmonary lobe, together with enlarged hilar and mediastinal lymph nodes (★ marked).
Figure 2 Positron emission tomography-computed tomography (PET-CT) images confirmed lung cancer in the right upper lobe (6.5 cm × 5.9 cm × 6.2 cm; SUV, 17.8) and mediastinal lymph node (2R) metastasis (1.6 cm; SUV, 10.4).

The patient preferred surgical treatment, and the primary tumor and involved lymph nodes were technically resectable. With the assessment of upfront multidisciplinary consultation, neoadjuvant crizotinib administration was selected to induce the downstaging and facilitate the subsequent surgical treatment. The patient proceeded to accept neoadjuvant targeted therapy with crizotinib (2×250 mg per day) from December 20, 2018 to March 4, 2019. Side effects were limited to grade 1 hepatic damage and mild edema of the lower limbs. Chest CT scan (March 10, 2019) after treatment showed that the lesion in the right upper lobe had obviously reduced to 29.3 mm × 28.3 mm, while the enlarged hilar and mediastinal lymph nodes had almost disappeared (Figure 3). Partial remission (PR) after neoadjuvant targeted therapy was achieved in the patient.

Figure 3 Computed tomography (CT) scan after crizotinib immunotherapy demonstrating the therapeutic effect as partial remission (PR). Tumor shrinked (A and B) and enlarged lymph nodes almost disappeared (B).

The patient underwent right upper lobectomy with mediastinal lymph node dissection in single-port video-assisted thoracoscopic surgery, 2 weeks (March 22, 2019) after the last dose. Following an uneventful postoperative course, the patient was discharged on postoperative day 6. Postoperative pathological examination revealed changes of moderately-to-poorly differentiated acinar-predominant adenocarcinoma of the lung after targeted therapy. The resected residual tumor was 35 mm × 35 mm × 32 mm, and the right upper paratracheal (2R), right lower paratracheal (4R), and right interlobar (11R) lymph nodes were involved. Immunohistochemistry examination revealed the following: TTF-1 (+), napsin A (partly +), CD56 (+), and KI-67 (50%+). Postoperative staging was confirmed as ypT2N2M0 (stage IIIA).

Given his good recovery situation and the risk of recurrence, the patient received adjuvant concurrent chemo- and radiotherapy. Four cycles of pemetrexed (860 mg on day 1, every 4 weeks) and cispla­tin (600 mg on day 1, every 4 weeks) were accepted. The postoperative radiotherapy was given at a dose of 1.8 Gy × 28 fx. During the combination therapy, the patient presented grade 1 hepatic damage which was relieved after symptomatic treatment. Treatment was completed by July 2019, and the compliance and tolerance of the patient appeared to be quite high (Figure 4). The patient was last followed up at the clinic 8 months after surgery (December 6, 2019), and no recurrence was observed (Figure 5).

Figure 4 Timeline of diagnosis and treatment.
Figure 5 Comparison of computed tomography (CT) scan after adjuvant chemoradiotherapy indicated no evidence of recurrence during the 8-month follow-up. Imagine of lung window (A) and imagine of vertical window (B).

Written informed consent was obtained from the patient for publication of this case report and any accompanying images.

Discussion and conclusion

Since ALK-TKI treatment was proven superior to chemotherapy for advanced ALK-positive NSCLC (9), the indication of ALK-TKI has tentatively been extended to the neoadjuvant therapy settings. In the case series report by Zhang et al. (4), crizotinib (250 mg twice per day) was administered to 11 patients with ALK-positive locally advanced NSCLC in the neoadjuvant setting. In total, 10 (90.9%) of 11 patients had a partial response, with the remaining patient experiencing stable disease response. Surgery was successfully performed on all patients after perioperative crizotinib, with 2 patients (18.2%) achieving pathologic complete response and 3 (27.3%) experiencing pathological node downstaging. Meanwhile, 6 patients had disease recurrence, and the median DFS time was 10.1 months (range, 5.3–20 months). Another case report showed that a patient with ALK-positive mediastinum-invasive NSCLC achieved radiological major response and pathological partial response after neoadjuvant crizotinib (10), with the DFS lasting 44 months as of publication. Previous trials demonstrate that neoadjuvant administration of crizotinib might be a promising therapy in providing an opportunity for resection and prolonged DFS for locally advanced ALK-positive NSCLC patients. The SAKULA trial was a multicenter single-arm phase II study aimed at assessing the efficacy and safety of neoadjuvant therapy with ceritinib followed by surgery in patients with ALK-positive resectable locally advanced NSCLC, with its preliminary results being reported in the 2019 World Conference on Lung Cancer . The ORR was 100% in the all 7 enrolled patients, and 6 out of 7 patients completed the full cycle of treatment and accepted surgery, with complete (R0) resection being achieved in 5 of them. Among the 7 evaluable patients, 4 patients achieved major pathologic response (57%, 95% CI: 18% to 90%) and 2 patients achieved pathologic complete response. Adverse events occurred in all the patients leading to 7 cases of dose interruption and 5 cases of dose reduction. The results revealed the efficacy of neoadjuvant ceritinib, but the optimal dose arrangement and further observation of safety require continued study.

In the case presented here, the patient achieved PR and pathological tumor downstaging in response to the neoadjuvant crizotinib, which suggests that locally advanced ALK-positive NSCLC patients may also benefit from minimally invasive surgery after the induction of targeted therapy. The administration of perioperative crizotinib provided a safe and efficacious chance for resection without increasing the difficulty and complications of the surgery. However, the duration of crizotinib therapy is still open to discussion as it concerns its optimal regimen. Considering that the effect of postoperative crizotinib remains unknown, we applied conventional chemotherapy and radiotherapy to achieve a better prognosis. Moreover, since the second-generation ALK inhibitors such as alectinib and brigatinib have been shown to be more effective than crizotinib in advanced NSCLC (11,12), their tentative application in the neoadjuvant setting is expected.

In conclusion, this is the first report of an ALK-positive stage IIIB NSCLC patient receiving neoadjuvant ALK-TKI treatment and radical minimally invasive surgery. This case demonstrated the safety and efficacy of crizotinib combined with minimally invasive surgery for the adjuvant setting. Further research focusing on neoadjuvant targeted therapy in locally advanced NSCLC can be expected to provide more substantive evidence and clarify the optimal regimen.


Funding: None.


Reporting Checklist: The authors have completed the CARE reporting checklist. Available at

Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at The authors have no conflicts of interest to declare.

Ethical Statement: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). Written informed consent was obtained from the patient for publication of this case report and any accompanying images.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See:


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Cite this article as: Tian Y, Huang J, Li C, Jiang L, Lin H, Lu P, Luo Q, Yang G. Perioperative crizotinib in a patient with stage IIIB ALK-positive non-small cell lung cancer: a case report. Ann Transl Med 2020;8(12):770. doi: 10.21037/atm-20-3927