Targeting ticagrelor: a novel therapy for emergency reversal
Review Article

Targeting ticagrelor: a novel therapy for emergency reversal

Brandon Cave1, Aranyak Rawal2, Devarshi Ardeshna3, Uzoma N. Ibebuogu4, Chittoor B. Sai-Sudhakar5, Rami N. Khouzam4

1Department of Pharmacy, Methodist University Hospital, Memphis, TN, USA; 2Department of Medicine-Pediatrics, 3College of Medicine, University of Tennessee Health Science Center, Memphis, TN, USA; 4Division of Cardiovascular Diseases, Department of Internal Medicine, University of Tennessee Health Science Center, Memphis, TN, USA; 5Department of Cardiac Surgery, University of Tennessee Health Science Center, Memphis, TN, USA

Contributions: (I) Conception and design: B Cave; (II) Administrative support: UN Ibebuogu; (III) Provision of study materials or patients: All authors; (IV) Collection and assembly of data: All authors; (V) Data analysis and interpretation: All authors; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.

Correspondence to: Brandon Cave. Pharm D, BCCP, ASH-CHC. Department of Pharmacy, Methodist University Hospital, 1265 Union Ave., Memphis, TN 38104, USA. Email: Brandon.cave@mlh.org.

Abstract: Newer P2Y12 inhibitors are prescribed in place of clopidogrel for patients with acute coronary syndrome (ACS) and are associated with significant bleeding risks. Currently, limited options exist for the management of life-threatening bleeding or acute reversal for patients on P2Y12 inhibitor therapy, specifically ticagrelor. Various interventions, including platelet transfusion and desmopressin, have been studied for ticagrelor reversal demonstrating limited success. PB2452 is a novel monoclonal antibody which binds to both ticagrelor and its active metabolite resulting in a rapid return of platelet aggregation. PB2452 has been studied in animal models and, most recently, in a Phase I trial in healthy volunteers. In animal models, PB2452 displayed rapid reversal of ticagrelor and its metabolites and return to near normal levels of platelet aggregation within 60 min. In healthy human volunteers, cohorts that received higher dose bolus and infusions of PB2452 over 12–16 h resulted in maximal and sustained reversal of ticagrelor inhibition of platelet aggregation. While it is currently not US Food and Drug Administration approved, future Phase 2 and 3 studies are currently underway that may lead to new directions for patients on ticagrelor therapy who require urgent reversal.

Keywords: Ticagrelor; PB2452; antiplatelet reversal emergency reversal


Submitted Jun 30, 2019. Accepted for publication Jul 29, 2019.

doi: 10.21037/atm.2019.08.08


Introduction

Potent P2Y12 inhibitors, prasugrel and ticagrelor, are routinely being prescribed in place of clopidogrel for patients with acute coronary syndrome (ACS) due to established superior efficacy in ACS with or without percutaneous coronary intervention (1-3). More potent platelet inhibition translates into an increased risk of bleeding. However, a net clinical benefit favors the use of these agents and has been incorporated in numerous guidelines (4-6).

In the Platelet Inhibition and Patient Outcomes (PLATO) trial, ticagrelor increased PLATO-defined non-CABG-related major bleeds (4.5% vs. 3.8%; HR 1.19, 95% CI, 1.02–1.38, P=0.03) compared to clopidogrel as part of a dual antiplatelet regimen (2). There was no difference in life-threatening or fatal bleeding between ticagrelor or clopidogrel-treated patients. However, ticagrelor-treated patients who experienced intracranial bleeding were less likely to survive the event compared to clopidogrel (0.1% vs. 0.01%; P=0.02). Although these events were rare, it may suggest that these events are more devastating and fatal with ticagrelor.

Ticagrelor is unique in that it reversibly binds to the P2Y12 receptor and requires shorter interruption compared to prasugrel (3 vs. 7 days) prior to surgical intervention (7). Frequently, patients receive dual antiplatelet therapy (DAPT) upon presentation with suspicion for non-ST segment elevation-ACS prior to knowledge of coronary anatomy, and if determined to be candidates for coronary artery bypass surgery, intervention is often delayed. Management strategies are complex in patients with hemodynamic instability, ongoing ischemia, critical coronary anatomy or high risk for recurrent ischemic events, as these are indications for emergent or urgent surgery without the luxury of waiting for full platelet recovery (4). Challenges also exist in patients with recent placement of a drug-eluting stent on DAPT with an urgent need for surgical intervention or invasive procedure, in which the risk of stent thrombosis is extremely high if administration of DAPT is temporarily discontinued or interrupted and bridging with intravenous cangrelor or GPIIb/IIIa inhibitors present a significant financial burden. At present there are limited options to address either urgent reversal for surgical procedures or life-threatening bleeding associated with P212 inhibitors, namely ticagrelor.

Current strategies for ticagrelor reversal

Current strategies to reduce the effects of antiplatelet drugs are limited. It has been suggested that platelet transfusion may be helpful, however, the mechanism of action of ticagrelor poses challenges with this strategy (8). The reversibility of ticagrelor’s inhibition of P2Y12 allows unbound ticagrelor and its active metabolite to inhibit fresh platelets (8). Reports highlight platelet transfusions, even at high-doses, have been unsuccessful in patients with intracranial hemorrhage previously treated with ticagrelor (9,10). In vitro studies have suggested that while pooled platelets are ineffective, platelet-rich plasma (PRP) may be more effective (8). The success of PRP is possibly attributed to the addition of plasma proteins, as demonstrated by an increase in platelet function with administration of human serum alone. Based on these results, it may be feasible to achieve similar effects with conventional doses of 20–40 g of human albumin to increase protein binding of ticagrelor.

Off-label use of desmopressin (DDAVP), a synthetic analogue of antidiuretic hormone, has been used in reversing ticagrelor-associated bleeding (11). DDAVP increases plasma factor VIII and von Willebrand factor concentration which promotes hemostasis (12,13). DDAVP may often be used as first-line treatment for patients with bleeding disorders and it has demonstrated efficacy in reversing bleeding related to heparin, aspirin and clopidogrel (14-16). A randomized crossover study of healthy volunteers on ticagrelor showed that DDAVP administration increased the primary hemostatic activity, and lowered bleeding time from 10.5 to 7.5 min, however, this difference was not statistically significant (11). DDAVP did not reverse the ticagrelor-associated inhibition of platelet aggregation. The results did not translate into clinical relevance. Without a definitive method of reversal, a specific antidote for ticagrelor may prove valuable as an agent for patients who require emergency procedures or have life-threatening bleeding. An antidote, human Fab MEDI2452, is currently under development and has demonstrable ticagrelor reversal in human in-vitro studies and mice in-vivo studies (17).


Pharmacology

PB2452 (formerly MEDI2452), a recombinant human monoclonal antibody antigen-binding fragment (Fab), has a dual mechanism of action in that it binds both to ticagrelor and its major active metabolite (AR-C124910XX) allowing restoration of platelet aggregation (17). It binds with strong affinity, approximately 100-fold greater than ticagrelor to its P2Y12 receptor. Adenosine triphosphate was used as the blueprint to develop PB2452. Despite structural similarity to adenosine, PB2452 remains highly target-specific and does not display the same binding affinity to adenosine or like compounds (17). PB2452 is only able to bind free ticagrelor and continues to bind ticagrelor as it dissociates from the P2Y12 receptor or plasma proteins. For this reason, sufficient time is necessary to achieve full reversal. Ticagrelor is primarily eliminated through the gastrointestinal tract. However, the complex of ticagrelor and PB2452 is eliminated primarily by the renal route. PB2452 represents the first in its class for reversing the effects of potent P2Y12 inhibitors.


Evidence of ticagrelor reversal

Animal models

PB2452 has been evaluated in several animal models, including mice and pigs. To simulate reversal of major bleeding, Swedish landrace pigs were pretreated with aspirin and ticagrelor followed by partial liver lobectomy (18). Pigs were treated with 250 mg of aspirin for two days prior to surgery and 10 mg/kg intravenously on the day of surgery. Ticagrelor was administered by a 60–min infusion of 3 mg/kg and terminated just prior to liver injury. Following surgical removal of partial lobe, a 600 mg/kg dose of PB2452 was administered over 5 min. PB2452 eliminated free ticagrelor and AR-C124910XX within 5 min and returned platelet aggregation to near normal in approximately 60 min, despite the experimental dosing regimen producing supratherapeutic concentrations of twice that of standard dosing in humans. Treatment with PB2452 showed a trend toward survival (70% vs. 44%) and survival time (240 vs. 169 min), however, neither of these achieved statistical significance due to the small population. Rate of mean arterial pressure decrease was significantly diminished in pigs that received PB2452, 0.08 vs. 0.141 mmHg per min in pigs without reversal. These trends suggest the feasibility of reversal and require further evaluation in human subjects.

Healthy volunteers

In a phase 1, randomized, double-blind, placebo-controlled trial, PB2452 was evaluated for safety and efficacy in healthy volunteers (19). The primary outcome evaluated was ticagrelor reversal assessed by platelet aggregation through light transmission aggregometry at various times prior to and after PB2452 administration. Additional secondary assessments of ticagrelor reversal were performed with a point-of-care P2Y12 assay, and a vasodilator-stimulated phosphoprotein (VASP) phosphorylation immunoassay. The primary safety endpoint evaluated was the frequency and severity of adverse events.

Healthy volunteers between the ages of 18 and 50 years, weight between 50 and 120 kg, and body-mass index between 18 and 35 were eligible for participation. Exclusion criteria included any contraindication to ticagrelor, predisposition to bleeding, or an estimated glomerular filtration rate <60 mL/min/1.73 m2. Randomization occurred in a 3:1 fashion of PB2452 and placebo into 10 cohorts (Table 1). A total of 64 volunteers were included of which 48 received PB2452 and 16 received placebo. Cohorts 1–3 were not given pretreatment with ticagrelor and given low doses of PB2452 over 30 min (0.1–1 g). Cohorts 4–10 were pretreated with ticagrelor and given larger, increasing doses of PB2452. Cohorts 4–6 were given loading doses of 1, 3 or 9 g over 30 min. Cohorts 7–10 received a total of 18 g of PB2452, in which loading doses and duration of infusions varied.

Table 1
Table 1 Treatment groups and results of PB2452 in healthy volunteers
Full table

The efficacy analysis was only conducted in those patients who received ticagrelor pretreatment (Cohorts 4–10). In volunteers that received placebo, platelet function returned to near baseline in 24–48 h. Cohort 4 had minimal response with 1 g of PB2452. Cohorts 5 and 6 achieved maximum ticagrelor reversal after the termination of the infusion at 30 min, however, this effect was sustained for only 1–2 h. Cohort 7 had sustained ticagrelor inhibition for 12 h with maintenance infusion, however, due to the lower loading dose, adequate onset was delayed for 2 h. Cohorts 8–10 were given 18 g administered over 12 h (Cohort 8) or 16 h (Cohorts 9 and 10) and with larger 6 g boluses at initiation. These regimens displayed maximal ticagrelor reversal within 5 min and sustained effect over 16–24 h by light transmission aggregometry, P2Y12 platelet-reactivity, and VASP assays. Additional tests for a rebound prothrombotic effect with PB2452 were negative.

Adverse events occurred more frequently in PB2452-treated patients (35% vs. 12%), with bruising at the injection site being the most common. There were no deaths, thrombotic events, infusion-related reactions, or dose-limiting toxic effects. PB2452 is a monoclonal antibody, and immunogenicity is a potential concern. Detectable antidrug antibodies were found in 44% of volunteers. However, only 12% became positive after receiving PB2452. Titers were low and did not show evidence of affecting safety or efficacy of PB2452. These results evaluating safety and efficacy are promising but will need confirmation in a larger trial of patients with coronary disease requiring cessation of antiplatelet therapies for surgery or with those active major bleeding.

Future development

At this time, PB2452 is not US Food and Drug Administration (FDA) approved; however, in 2019, the FDA granted Breakthrough Therapy designation for PB2452 based on Phase 1 and preclinical studies. Breakthrough Designation will allow for expedited development, review, communication and guidance from the FDA and future potential of Accelerated Approval and Priority Review (20). Recruitment to begin Phase 2a study in healthy older and elderly patients (aged 50–80 years) to evaluate safety, tolerability and pharmacokinetics and pharmacodynamics is currently underway (NCT03928353) (21).


Conclusions

Despite the routine use of potent P2Y12 inhibitors and investment into reversal therapies for novel anticoagulants, there is a lack of therapeutic options to effectively reverse antiplatelet therapy. This unmet need may continue to grow as combinations of anticoagulation and antiplatelet therapies increase bleeding risk when indications overlap. Although rare, the consequences of a major bleed from ticagrelor can be severe, and platelet infusions are ineffective. The addition of a reversal agent for ticagrelor will increase the safety of patients requiring reversal for acute uncontrolled or life-threatening major bleeding events. PB2452 represents an advancement in this area and is the first reversal agent of its kind in the case of urgent surgical procedures or uncontrolled major or life-threatening bleeding.


Acknowledgments

None.


Footnote

Conflicts of Interest: Dr. Cave discloses that he serves on the speakers bureau for Portola Pharmaceuticals. The other authors have no conflicts of interest to declare.

Ethical Statement: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.


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Cite this article as: Cave B, Rawal A, Ardeshna D, Ibebuogu UN, Sai-Sudhakar CB, Khouzam RN. Targeting ticagrelor: a novel therapy for emergency reversal. Ann Transl Med 2019;7(17):410. doi: 10.21037/atm.2019.08.08